Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXIX, Issue 1
Written by Emoke PALL, Mihai CENARIU, Simona CIUPE, Cristian CRECAN, Ioan Stefan GROZA
Mesenchymal stem cells (MSCs) are harvested after birth; they are adult stem cells and, due to their unique potential, are considered very valuable tools for equine regenerative medicine. MSCs have self-renewal capacity and multilinear differentiation potential. Multiple protocols are used to induce the directed differentiation of these cells. The aim of our study was to evaluate the osteoinductive potential of a glycoprotein from the transferrin family, lactoferrin (Lf) on MSCs isolated from equine synovial fluid. The cell line (syMSCs) used for this study was obtained from synovial fluid samples from a healthy horse. The isolated cells were characterized morphologically, immunophenotypically and functionally respecting the standards of the International Society for Cell Therapy which were originally drawn up for human MSCs (cellular plastic adherence, expression of specific surface markers and trilinear differentiation capacity). The cells were cultivated in normal propagation medium for MSCs. For osteogenic differentiation, syMSCs were seeded at a concentration of 1 × 105 cells/3 mm well, and cultured in osteogenic induction medium with (3 different concentration: 20, 50, 100 μg/mL) and without Lf. The proliferation potential of the cells were assessed using CCK8 assay and the markers of osteogenic differentiation (alkaline phosphatase, ALP) were detected using fluorimetric assay. Our results demonstrate the osteogenic potentiation capacity of human lactoferrin correlated with concentration, thus our future studies will try to elucidate the osteoinductive mechanism of lactoferrin by applying genomics and proteomics techniques.
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