Scientific Works Series C. Veterinary Medicine

PRINT ISSN 1222-5304, ISSN-L: 2065-1295, ISSN CD: 2343-9394,ISSN ONLINE 2067-3663
 

Frz OPERON AND R4 GENE VIRULENCE PRESENT IN ROMANIAN APEC (AVIAN PATHOGENIC ESCHERICHIA COLI) ISOLATES

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXVII, Issue 2
Written by Maria Rodica GURĂU, Hasan Majid HAMEED, Fănel OȚELEA, Dragoș COBZARIU, Doina DANEȘ

Escherichia coli (E. coli) infection has a major effect on poultry production, the numerous clinical forms making it the most frequently reported diseases in commercial flocks of chickens but also in turkeys and pigeons. Some Avian pathogenic E. coli (APEC) strains, may share virulence factors with human extraintestinal pathogenic. The data presented in this study complete the characterization of the pathotypes of 13 APEC strains, previously isolated and characterized (Gurau M.R. et al., 2018, 2020), with the investigation over the presence of Frz operon and R4 virulence gene, by PCR method. Amplification was performed in two different runs, one for each gene, the temperature protocols being different. The Frz operon was identified in 11 isolates (84.61% or 11/13) and R4 was identified in 4 isolates (30.76% or 4/13). These results, correlated with those previously reported, highlighted that Frz operon and R4 genes are independently expressing their virulence.

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