Scientific Works Series C. Veterinary Medicine

PRINT ISSN 1222-5304, ISSN-L: 2065-1295, ISSN CD: 2343-9394,ISSN ONLINE 2067-3663
 

ANTIGEN EXPRESSION ENHANCEMENT OF Mycobacterium bovis AN5

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXVII, Issue 2
Written by Horia DINU, Elena NEGRU, Anca BULGARU, Dragoş LUPU, Mihai DANEŞ, Doina DANEŞ

The revealing value of the purified protein antigens designed for the bovine tuberculosis screening registers considerable variations etween the different batches of product, even in the case of the same obtaining protocol and of the same operator. In order to limit variations in the potency of the purified protein extract and to improve the expression of immunodominant proteins, ifferent culture protocols have been designed, which, under reproducibility conditions, could reduce the variation limits between atches. The study of the genetic profile identified the AN5 strain used as a virulent Mycobacterium bovis strain belonging to the bovis-caprae ineage. The potency of the purified protein extract was quantified by testing dilutions on guinea pigs. The metabolism modulation of he studied strain, by alternative culture conditions, improved its antigens profile, these being the key compounds for the potency of he tuberculin.

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