Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXIV, Issue 1
Written by Georgi STOIMENOV, Gabriela GOUJGОULOVA, Georgi GEORGIEV, Chavdar FILIPOV, Roman PEPOVICH, Kalin HRISTOV, Branimir NIKOLOV
The aim of our study was to assess the possibility of serological tests for a detection of antibodies against Influenza A virus in wild migratory, zoo birds and alive birds, presented on markets. The samples were collected in Bulgaria during the epizootic in 2015. Totally 209 specimens have been tested, of which 179 only by ELISA and 30 both by ELISA and HI assay. Some differences during the testing of two yolk sacs of eggs, from the found death Dalmatian pelicans, have been demonstrated, where ELISA and AGID were negative, but HI was positive; the following VNR found them to bepartially positive. A possible explanation for the observed contradiction could be given by the specific haemagglutinin, located on the surface of viral particle. The obtained positive serum samples of wild birds from Sofia Zoo and those from a market for alive birds have shown that, the supervision of Avian influenza should not be focused only on the migratory birds, because the disease can be introduced by an import of exotic birds and their offer through auctions and markets.
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