Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by János DÉGI, Iulia BUCUR, Voichiţoiu Robert VILI, Viorel HERMAN
Colonization with Staphylococcus aureus resistant to methicillin (MRSA) has recently been identified in pigs and that the people working in these sectors and increasing concern about the role of pigs as reservoirs of MRSA infections in humans were reported more frequently in the literature. The research had as purpose to determine the frequency of staphylococcal strains isolated from pig herds, phenotypic characterization of isolates and identification of methicillinresistant strains and types of resistance. Samples were taken from clinically healthy pigs from a swine farm in Arad, from October to December 2013. Samples required bacteriological examinations were collected from a total of 87 clinically healthy pigs. After growth, staphylococcal isolates were identied according to their characteristics as outlined in Bergey’s Manual of Determinative Bacteriology and the Manual of Clinical Microbiology. From pig farm were isolated 28 strains of staphylococci, including 20 coagulase positive strains (CoP, represented by S. hyicus and S. aureus) and 8 coagulase negative strains (CON, represented by S. haemolyticus, S. epidermidis respectively S. sciuri), isolated from clinically healthy pigs in different anatomical areas. All strains of staphylococci isolated from pigs showed sensitivity of 100% for novobiocine, rifampicine, pristinamycin, ciprofloxacin, vancomycin, ceftriaxone, cefoxitin, cefaclor and ampicillin/sulbactan, considered the drug of choice for these bacteria. As against used β-lactams (methicillin, ceftriaxone, cefoxitin, cefaclor, ampicillin with sulbactan) antibiotic sensitivity was highest, except methicillins, which were isolated 4 resistant strains, two S. hycus methicillin-resistant strains and two S. aureus strains. After testing staphylococci strains isolated from pigs, against 17 antibiotics were identified methicillin-resistant strains and several types of resistance to β-lactams, tetracyclines, macrolides and polymyxin B.
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