Published in Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 4
Written by Stănescu (Pascal) M., Bîrţoiu, I., Deleuze, S.
The fertilizing capacity of dog spermatozoa depends on many factors, like: motility, plasmatic membrane integrity (viability), acrosome integrity. The role of the prostatic fluid in the fertilization process is still controversial. The aim of our study was to evaluate the effect of post-thaw dilution with autologous prostatic fluid on viability, motility and acrosome status of cryopreserved dog spermatozoa. Semen was collected from 6 Beagle dogs. The sperm rich fraction was frozen with a standard extender for dog semen containing Tris, fructose, glycerol and egg yolk (TFG-EY). For each dog, six straws were thawed: three straws were diluted 1:2 with autologous prostatic fluid, while the others were not diluted at all. Motility (CASA), viability and acrosome status (flow cytometry), morphology (Diff-Quick stain) were assessed at 5 minutes, 1 hour and 2 hours post-thaw (T0, T1, T2). There were no significant differences regarding the morphology of fresh and frozen semen. The addition of prostatic fluid significantly reduced the total and progressive motility and increased the percentage of reacted acrosomes at T0, T1 and T2 (P < 0.05). Although the addition of prostatic fluid did not affect the viability and the morphology of frozen-thawed semen, it reduced the motility and increased the percentage of acrosome reactions.
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cryopreservation
