Published in Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 4
Written by Groza I., Cătană Laura, Pall Emoke, Cenariu M., Pop Daria, Ilea Cristina
Interest of the medical world towards regenerative therapy using mesenchymal stem cells has become increasingly prominent, given the many recent successes. Dogs are ideal candidates for testing the methods of isolation, cultivation and differentiation of mesenchymal stem cells into multiple cell lines and their use in regenerative therapy. The aim of this paper was to test the multipotence and multilinearity of mesenchymal stem cells derived from canine bone marrow and umbilical cord blood. Mesenchymal stem cells were obtained from a total of 12 dogs following medular aspiration or by collecting cord blood during caesarean section. Samples were processed using Histopaque 1077 and then cultured in α-MEM supplemented medium. In order to assess the stemness and multipotency of mesenchymal cells isolated from canine bone marrow and umbilical cord blood, their phenotype was characterized by assessing the Oct4 gene expression followed by the evaluation of their differentiation potential towards bone, cartilage, fat and nerve cells. Canine bone marrow and umbilical mesenchymal stem cells expressed the Oct4 gene. This gene expression was not identified after differentiation, however was shown in cells grown in propagation medium. Osteogenic, chondrogenic, adipos and nervous differentiation was demonstrated by identifying specific morphology, specific stainings and by assessing the gene expression of genes of interest. Canine mesenchymal stem cells have a high multilineage capacity, being able to differentiate towards osteogenic, chondrogenic, adipogenic and nervous lines, These properties can be exploited in order to use this type of cell therapy in homologous, heterologous and even xenogenic regenerative therapies.
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