Published in Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 3
Written by Pall Emoke, Ciupe Simona, I.S.Groza, Cenariu M., Niculae Mihaela, Alexandra Roman
Stem cells isolated from various tissues can self-renew and produce different cell types. Oral cavity may be a valuable source of mesenchymal stem cells (MSCs) that can be isolated and expanded in vitro, providing a unique reservoir of stem cells from accessible tissue resources. The aim of this study was to isolate and assess the multipotent characteristics of MSCs dog and human in order to cover gingival recessions in future with these cells. Human and dog MSCs were obtained from palatal subepithelial connective tissue, expanded and cultured in DMEM/F12 culture medium supplemented with 10% fetal calf serum (FCS) and antibiotics. The cell surface phenotype of the presumed palatal MSCs was characterized at the 4th passage. All flow cytometry measurements were made using a FACS Canto II flow cytometry system (BD Biosciences, San Jose, CA, USA) and analysed using the DIVA program. Our data confirmed that the isolated and cultivated dog and human MSCs cells have multipotent character based on specific surface antigen expressing (CD44, CD34/45, CD29).
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connective tissue
