PRINT ISSN 1222-5304, ISSN-L: 2065-1295, ISSN CD: 2343-9394,ISSN ONLINE 2067-3663
 

COMPARISON BETWEEN AN AUTOMATIC AND A MANUAL PROTOCOL FOR FREEZING CANINE SEMEN

Published in Scientific Works. Series C. Veterinary Medicine, Vol. 19 ISSUE 3
Written by Manuela PASCAL, Ruxandra COSTEA, Alin Ion BÎRTOIU

For the freezing of canine semen, slow to fast cooling rates have been used. Today, there are many ways of achieving this by using manual protocols, automatic protocols or ultrafreezers. Straws were divided in two groups: one batch (10 ejaculates) was automatically frozen, while the other batch was frozen manually. Motility (computer assisted sperm analyzer), morphology and acrosome status (Spermac® stain) were evaluated for fresh and frozen-thawed semen. The manual freezing protocol provided higher total (23.85%) and progressive motility values (20.41%) compared to the automatic protocol (13.26 % total motility, progressive motility 9.90%). The acrosome status was strongly influenced by the cryopreservation process, but there were n significant differences between the two ptotocols.When using the CaniPro Freeze® extender, a slower cooling rate (the manual protocol) gave better results then a fast one (the automatic protocol)

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