PRINT ISSN 1222-5304, ISSN-L: 2065-1295, ISSN CD: 2343-9394,ISSN ONLINE 2067-3663
 

SALMONELLA IDENTIFICATION BY COMPARING BACTERIOLOGICAL AND MOLECULAR METHODS

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LX (1)
Written by Zorița Maria COCORA, Laurențiu Marcel PANDELE, Petru Mihai COCORA, Ioan ȚIBRU

This present study attempts comparing classical method ISO 6579:2002 with a quick, polymerase chain reaction (PCR) for the identification and isolation of Salmonella carrier pigs without clinical signs by collecting feces and sanitation tests on the surface of carcasses. After analyzing the samples (n=51) by the two methods (Bacteriological and PCR) has resulted a higher number of positive samples (43.13%) by PCR, compared to the bacteriological method, where only 35.29% were positive samples. After sequencing and introduction into the gene pool (GeneBank), it was found that the most common serovars isolated from the feces were S. Typhimurium, S. Newport, from the surface of carcasses were S. Typhimurium, S. Typhi, S. Newport and S. Enteritidis serovars, and on the surface of working equipment S. Typhimurium and S. Agona.

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