PRINT ISSN 1222-5304, ISSN-L: 2065-1295, ISSN CD: 2343-9394,ISSN ONLINE 2067-3663
 

ISOLATION AND IDENTIFICATION OF PSEUDOMONAS AERUGINOSA STRAINS PRODUCING β-LACTAMASES (ESBL) AND CARBAPENEMASES (MBL) OF HUMAN ORIGIN

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by Oana Alexandra CIOCAN, Mihai CARP CĂRARE, Andreea Paula COZMA, Cătălin CARP–CĂRARE, Cristina RÎMBU, Gabriela COMAN, Elena PETRARU, Carmen–Valentina PANZARU

The community of medical veterinarians and humans draw attention to the microorganisms with potential pathogens that are both common and multiresistant to the latest antibiotics. Bacteria common to both animals and people favor the cross transmission of these strains with a major public health risk. Pseudomonas aeruginosa, a motile, nonfermenting Gram-negative bacterium, is an important opportunistic animal and human pathogen that causes acute and chronic infections in immunocompromised patients. Pseudomonas aeruginosa has acquired several mechanisms of resistance to multiple groups of antibiotic agents. The isolation and identification of Ps. aeruginosa producing ESBL and MBL may often be challenging to microbiology laboratories, the level of expression of β-lactamases and MBL can affect the performance of phenotypic tests, and the lack of synergy might be due to a very high level of expression overcoming the effect of the inhibitors resulting in falsenegative results. Ps. aeruginosa from different purulent collections has an atypical appearance, even a specific pigment that is not present for all the strains. These investigations are part of a larger research study, aimed at highlighting the strains of Pseudomonas aeruginosa common resistance genes in humans and animals. In this study, 93 Ps. aeruginosa strains were collected from pediatric patients. In order to establish resistance, profiling samples were isolated and identified for the production of β- lactamase medium Brilliance ESBL AGAR (Oxoid) and for the production of MBL was tested Imipenem with EDTA.

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