PRINT ISSN 1222-5304, ISSN-L: 2065-1295, ISSN CD: 2343-9394,ISSN ONLINE 2067-3663
 

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LX (1)
Written by Tandzhu MEHMEDOV, Branimir NIKOLOV, Geno ANGELOV

The effect of the application of probiotics Laktiferm Basic® 300 and Laktina®, and antibiotics Colivet Oral powde and Pharmastim 8 %® at pheasants were compared. We explored the possibility of use of probiotics for the prevention and treatment of E. coli infections in pheasants aged from 1 to 30 days. In the experiment take part 5 groups pheasants infected with enteropathogenic strain of E.coli O 103 in an amount of 108 cfu / ml ( positive control - treated with Colivet Oral powde, negative control and three test groups with: Laktiferm Basic 300®, Laktina® and Pharmastim®). Survival, mortality and post-mortem lesions in infected with E. coli pheasants of all groups were studied.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LX (1)
Written by Andra ENACHE, Pip BOYDELL, Iuliana IONAȘCU, Alexandru ȘONEA

Uveal cysts are fluid filled structures arised from the iris or ciliary body commonly presented in certain breeds such as Golden retriever, Labrador retriever and Boston terrier. This report describes the clinical signs, the laser technique and the postoperative aspect for a Flat Coated Retriever with iris cysts. A 6 year-old, neutered male Flat Coated Retriever was referred to the Animal Medical Centre with a history of two pigmented masses in the anterior chamber. Ophthalmic examination of the right eye showed the presence of two highly pigmented iris cysts attached at the pupillary margin to the posterior iris. Nonsteroidal eye drops (ketorol tromethamine) and a course of oral meloxicam were initiated a week before the procedure. Under general anaesthesia the diode laser was used with an indirect ophthalmoscope headset and a 20 diopter lens. Preoperative atropine and proxymetacaine eye drops were applied. Diode laser was used to deflate the cysts. Initial pigment dispersion in the anterior chamber and on the surface of the lens was noted. Topical nonsteroidal, steroidal and antibiotics (dexamethasone, polymyxin and neomycin) eye drops were continued to control the secondary uveitis. Clinical progress was monitored and there was minimal postoperative aqueous flare with no ocular discomfort. Diode laser is an effective treatment of highly pigmented iris cysts.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LX (1)
Written by Ivan PAVLOVIC, Snezana IVANOVIC, Bozidar SAVIC, Dragica VOJINOVIC

Today, breeding of small flocks of sheep and goats has increased on Belgrade area. Alongwith the increasing number of flocks the interest in examination of their health status increased as well. A study about occurrence of Paramphistomidae trematodes in small ruminants at spread Belgrade area was conducted. During the period from March 2009 to January 2010a total of 91 flocks of goats (n = 22) and sheep (n = 69), from 6 Belgrade districts, were examined. Paramphistomidae parasites were foundin 7 out of 67goats (prevalence of 10.44%) and 12 out of 89 sheep (prevalence of 13.48%) examined by necropsy.. After histological examination, the paramphistome trematodes were identified as Paramphistomum cervi. P.cervi has been previously found in sheep and cattle in Serbia, but this is the first report of P.cerviin in goats.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LX (1)
Written by Stelian BĂRĂITĂREANU, Doina DANEȘ

Emergence of “novel” infectious agents with or without zoonotic potential continues to occur in livestock. Such events have many causes, some natural and a lot off are associate with human interference upon microorganisms or their environment. Animal world is hosting many more pathogens than those who are subject of current surveillance and/or diagnostic: some of them are endemic in limited ecosystems, and are usually defined as exotic pathogens by European countries (e.g., Hendra virus, Nipah virus, Akabane virus); others are not associated with collective or individual known pathology in them natural hosts (asymptomatic carriers) but can produce or can be involved in diseases of other domestic animals or humans (e.g. Hanta virus, Crimea-Congo hemorrhagic fever virus), or are just new discovered pathogens (e.g., Schmallenberg virus). It is difficult to accomplish, but it would be highly useful to investigate if these organisms, introduced in different populations other than the originating one, could generate pathology. Would be useful to map the distribution of these newly discovered agents whose potential pathogen is still unevaluated or even appear devoid of pathogenicity, to estimate their emerging potential in the case of contact with unusual hosts. The large number of pathogens, which are not subject to official notification, makes difficult their active surveillance by specific laboratory testing methods; the cost-effectiveness of active surveillance systems could not be accepted for the animal disease surveillance and the prompt reporting. However, the passive surveillance and the risk analysis (exposure assessment and risk characterization) can be perform using conventional or participatory epidemiology if the specialist or farmers are trained to identify the pathology that can be produced by these new pathogens. The aim of this review was to describe the main clinic pathological features generated in livestock by “novel” infectious agents: Schmallenberg virus, Hendra virus, Menangle virus, Nipah virus and Usutu virus. In our opinion, once emerged a new microorganism it is advisable to make investigation in similar ecosystems to check his presence.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LX (1)
Written by Nikolay SANDEV, Darinka ILIEVA, Nikolina RUSENOVA, Emil ILIEV

Analysis of the results from the serological study of bovine enzootic leukosis (BEL), conducted in 2012 with commercial ELISA kits, showed a wide prevalence of infection in Bulgaria (33.38% on average) and over 2000 outbreak locations nationwide. Within districts, the percentage of infected animals varied between 13.05% and 63.85%. As a result of mandatory measures of prevention and treatment of BEL, included in the national eradication programme, the infection has been eradicated in one district and 78 outbreak locations.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LX (1)
Written by Ionela Denisa SORESCU, S. MORARIU, I. OPRESCU, Narcisa MEDERLE, M. S. ILIE, Ionela HOTEA, GH. DĂRĂBUȘ

This study was conducted to determine infection with endoparasites, in dogs from Timiș County and to analyze the potential risk factors that support this infection. Study was conducted in October 2009 – April 2011. There were collected 183 faecal samples. The examination of the samples was accomplished using flotation method (Willis), direct smear examination using Lugol solution and by rapid tests (Giardia Antigen Test Kit IDEXX). Samples were collected from dogs from shelters. Parasitic fauna of dogs was represented by Giardia spp. (24.59%), Toxocara spp. (4.37%), Ancylostoma/Uncinaria spp. (1.63%), Trichocephalus spp. (0.54%) identified as single parasites. Also, associations of parasites were identified. Age up to six months is an important risk factor. The breed and gender did not represent any considerable risk factors. Of the dogs with Giardia spp., 76.92% reported symptoms and 23.07% were asymptomatic.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LX (1)
Written by Alexander ATANASOFF, Lilko DOSPATLIEV, Dimitrinka ZAPRYANOVA, Cigdem ONER

Alexander ATANASOFF, Lilko DOSPATLIEV, Dimitrinka ZAPRYANOVA, Cigdem ONERProblem statement: Snail`s meat is consumed as a delicate product in countries such as France, Greece, Italy and many others. The present paper is aimed to determine the protein, moisture, ash, fat, as well as the mineral content of snail`s meat. Organisms: 400 species of snail (Helix lucorum). Approach: The content of protein, fat and ash and concentrations of iron, potassium, sodium, calcium, phosphorus, magnesium, copper, selenium and zinc were determined by automatic systems and electro thermal atomic absorption spectrometry (ETAAS) after microwave digestion. Mean values and their respective coefficients of variation were calculated from the measured concentrations. The results from the analysis showed that snail meat is rich in protein (18.56%) and low in both ash (1.61%) and fat (1.40%). The major minerals found in this study were calcium (159.3 mg/100 g), phosphorus (102.2 mg/100 g), potassium (94.3 mg/100 g), sodium (87.6 mg/100 g) and magnesium (38.0 mg/100g). However, iron, zinc, manganese and copper content were less than 10 mg/100 g. Conclusion: The results of this study have showed that snails meat (Helix lucorum) are good sources of protein and micro elements and that its consumption can promote health, proper growth and development of the human body.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LX (1)
Written by Alina Maria IONESCU, Gheorghe Valentin ROMAN

The paper present the results of the research made in the years 2007-2009 regarding chemical composition and nutritional value of some oilseeds crops promoted in organic agriculture: safflower, camelina, oil pumpkin and oil flax. The biochemical compounds (glucides, proteins, lipids and minerals) have been analyzed by using the chemistry laboratory methods: for glucides, Bertrand Method; for proteins, Kjeldahl Method; for lipids, Soxhlet Method; for minerals, Spectrophotometer Method. In average, the chemical composition of these crops cultivated in Moara Domneasca Experimental Field was the following: for safflower – 12.60% proteins, 28.37% lipids, 46.41% glucides, 3.60% minerals, and the energetic value was 505.78 kcal; for camelina – 20.29% proteins, 31.68% lipids, 36.28% glucides, 4.29% minerals, while the energetic value was 526.63 kcal; for oil pumpkin – 29.50% proteins, 36.92% lipids, 18.50% glucides, 5.41% minerals, and the energetic value was 540.15 kcal; for oil flax - 22.56% proteins, 34.10% lipids, 27.73% glucides, 5.25% minerals, while the energetic value was 558.45 kcal. The study of the nutritional value of these oilseeds crops in organic agriculture conditions evidenced the very special role which should they occupy in the development of biodiversity, environmental protection and diversification of food.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LX (1)
Written by Kalin HRISTOV, Parvan PARVANOV, Borislav KASHAMOV, Roman PEPOVICH, Branimir NIKOLOV

The study included 120 randomly selected lactating goats of the Bulgarian breed dairy and local cross. The animals were housed in two licensed farms in Bulgaria, under the same technological systems. Goats are grouped in to treatment groups according to age and number of lactation periods. The study aims to clarify the impact of the number of lactation and age as a factor in the spread of subclinical mastitis. Sterile milk samples have been taken of all the animals for bacteriological status and for the performance of physical and chemical analysis. The results show a clear trend of increasing intramammary infection with age and raise in number of lactation, while caprine animals aged 2-3 years, the affected dairy halves are 20%, while those age dover 8 years affected halves are 56.7 %. Isolated pathogens are mainly from the group of coagulase-negative staphylococci.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LX (1)
Written by Zorița Maria COCORA, Laurențiu Marcel PANDELE, Petru Mihai COCORA, Ioan ȚIBRU

This present study attempts comparing classical method ISO 6579:2002 with a quick, polymerase chain reaction (PCR) for the identification and isolation of Salmonella carrier pigs without clinical signs by collecting feces and sanitation tests on the surface of carcasses. After analyzing the samples (n=51) by the two methods (Bacteriological and PCR) has resulted a higher number of positive samples (43.13%) by PCR, compared to the bacteriological method, where only 35.29% were positive samples. After sequencing and introduction into the gene pool (GeneBank), it was found that the most common serovars isolated from the feces were S. Typhimurium, S. Newport, from the surface of carcasses were S. Typhimurium, S. Typhi, S. Newport and S. Enteritidis serovars, and on the surface of working equipment S. Typhimurium and S. Agona.

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